The Influence of Adipocyte Secretome on Selected Metabolic Fingerprints of Breast Cancer Cell Lines Representing the Four Major Breast Cancer Subtypes

Molecular subtype (MS) is one of the most used classifications breast cancer (BC). Four MSs are widely accepted according to receptor expression estrogen, progesterone, and HER2. The impact adipose tissue on BC MS metabolic impairment still unclear. present work aims elucidate alterations in cell lines representing different subjected adipocyte associated factors. Preadipocytes isolated from human subcutaneous were differentiated into mature adipocytes. representative exposed secretome. Extracellular medium was collected for metabolomics RNA extracted evaluate enzymatic by RT-PCR. Adipocyte secretome exposure resulted a decrease Warburg effect rate an increase cholesterol release. HER2+ (BT-474 SK-BR-3) exhibited similar pattern, contrast luminal A (MCF-7) triple negative (TN) (MDA-MB-231), both presenting identical metabolisms. Anaplerosis found TN cells, whereas cataplerotic reactions likely occur lines. Our results indicate that affects central metabolism distinctly each line.